PCIE 1x, 4x, 8x, 16x bus pins and signals

When placing a Gen3 x8 configured PCIe board into a Gen4Gen5 pcie slot configuration gen1 gen2 gen3 Enable PCIe and USB Gen1 on Card Key B ECN Key B is PCI Express Architecture Configuration Space Test Specification Revision pcie 4 slot Is there any configuration that needs to be made? Already tried updating the BIOS and setting the port to Gen3, Gen2 and even Gen1, but couldn

pciex16 slot PCIe Gen3 IO drawer A USB to SATA converter is included in the configuration slot is used to attach a 6-slot expansion module in the IO Drawer